Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart

Osteoarthritis (OA) is a degenerative joint disease accompanied by pain and loss of function. Adipose tissue harbors mesenchymal stem/stromal cells (MSC), or medicinal signaling cells as suggested by Caplan (Caplan, 2017), used in autologous transplantation in many clinical settings. The aim of the study was to characterize a stromal vascular fraction from microfragmented lipoaspirate (SVF-MLA) applied for cartilage treatment in OA and compare it to that of autologous lipoaspirate (SVF-LA). Samples were first stained using a DuraClone SC prototype tube for the surface detection of CD31, CD34, CD45, CD73, CD90, CD105, CD146 and LIVE/DEAD Yellow Fixable Stain for dead cell detection, followed by DRAQ7 cell nuclear dye staining, and analyzed by flow cytometry. In SVF-LA and SVF-MLA samples, the following population phenotypes were identified within the CD45⁻ fraction: CD31⁺CD34⁺CD73^±CD90^±CD105^±CD146^± endothelial progenitors (EP), CD31⁺CD34⁻CD73^±CD90^±CD105⁻CD146^± mature endothelial cells, CD31⁻CD34⁻CD73^±CD90⁺CD105⁻CD146⁺ pericytes, CD31⁻CD34⁺CD73^±CD90⁺CD105⁻CD146⁺ transitional pericytes, and CD31⁻CD34⁺CD73^highCD90⁺CD105⁻CD146⁻ supra-adventitial-adipose stromal cells (SA-ASC). The immunophenotyping profile of SVF-MLA was dominated by a reduction of leukocytes and SA-ASC, and an increase in EP, evidencing a marked enrichment of this cell population in the course of adipose tissue microfragmentation. The role of EP in pericyte-primed MSC-mediated tissue healing, as well as the observed hormonal implication, is yet to be investigated.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627838/